Casa / liquid detergent and alginate lyase What Are Enzyme Detergents and How Do They Work? - …- liquid detergent and alginate lyase ,Not only are enzymes natural and readily biodegradable, they allow us to clean, brighten, and whiten smarter. For instance, the enzyme cellulase helps take brightness one step further than bleaching agents by not just covering up soils but by physically removing them. Cellulases cleave off damaged micro fibrils and release any captured dirt ...A rapid, sensitive, simple plate assay for detection of ...2015-9-1 · The alginate lyase activities of the microbial strains indicated by both methods (CPC and Gram's iodine) were calculated as alginate-lyase-activity area using the formula πD 2 /4, where D is zone diameter in mm for total zone area due to enzyme activity. In both methods, S. degradans displayed bigger zones due to greater amount of alginate lyase activity followed by …
Liquid-chromatographic analysis of the depolymerization …
1986-10-1 · The extracellular alginate lyase activity from a fermentative marine bacterium isolated from actively growing tissues of Sargassum fluitans has been purified and studied with respect to substrate specificity and mechanism. The enzyme endolytically depolymerizes (1→4)-β-d-mannuronan derived from alginate to oligomeric products possessing 4,5-unsaturated, …
contact supplierWhatsappAlginate lyase: Review of major sources and classification ...
2022-3-21 · Introduction. Alginate is the most abundant polysaccharide (about 40% of dry weight) of brown algae, which consists of β-D-mannuronate (M) and α-L-guluronate (G) as monomeric units. 1 These units are linked in 3 kinds of different blocks, poly β-D-mannuronate (polyM), poly α-L-guluronate (polyG) and the heteropolymer (polyMG). 2 Some bacteria can also synthesize …
contact supplierWhatsappScreening of alginate lyase-excreting microorganisms from ...
2017-4-4 · Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum …
contact supplierWhatsappStructural and Functional Studies on Three Alginate Lyases ...
2020-11-12 · Keywords:Alginate lyase, chimeric protein, ESI-MS, NMR, site-directed mutagenesis, substrate specificity. Abstract:To date, several microorganisms that can metabolize alginate have been characterized. In these microorganisms, a class of enzymes called alginate lyases (Alys) catalyze the depolymerization of alginate into oligomers via an ...
contact supplierWhatsappDetection and Pharmacokinetics of Alginate ...
2018-6-14 · Preparation of AOs. Sodium alginate (5g) was gradually dissolved in 500ml of distilled water with heating at 50 C, and an aliquot (1ml) of alginate lyase solution (500mg/ml) was added to the alginate solu-tion. The reaction mixture was incubated at 40 C for 3d. During the incubation period, the same volume of fresh enzyme solution was added 3 ...
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2018-3-9 · Alginate oligosaccharides with different bioactivities can be prepared through the specific degradation of alginate by alginate lyases. Therefore, alginate lyases that can be used to degrade alginate under mild conditions …
contact supplierWhatsappIsolation of a novel alginate lyase‐producing Bacillus ...
2016-7-20 · The polyM‐specific alginate lyase in this study showed optimal temperature of 50°C (Fig. 2A), whereas the optimal temperature of most alginate lyase is below 45°C (Li et al., 2011). Our study suggests that the M3 lyase is a new and unique member in alginate lyase family due to its higher optimal temperature and substrate specificity.
contact supplierWhatsappScreening of alginate lyase-excreting microorganisms from ...
2017-4-4 · Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum …
contact supplierWhatsappPurification and characterization of alginate lyase from ...
2018-9-1 · Alginate is a linear hetero-polyuronic acid polymer that is made up of β-d-mannuronate (M) and its C5 epimer α-l-guluronate (G).Due to its arrangement, alginate can be classified in three ways: poly-β-d-mannuronate (polyM), poly-α-l-guluronate (polyG), and heteropolymeric regions 1, 2, 3.Due to their high viscosity and gelation, alginate polymers are widely used in …
contact supplierWhatsappThree Alginate Lyases from Marine Bacterium …
2010-12-19 · Three alginate lyases (A, B, and C) from an alginate-degrading marine bacterium strain HZJ216 isolated from brown seaweed in the Yellow Sea of China and identified preliminarily as Pseudomonas fluorescens are purified, and their biochemical properties are described. Molecular masses of the three enzymes are determined by SDS-PAGE to be 60.25, 36, and …
contact supplierWhatsappCharacterization of Alginate Lyase Activity on Liquid ...
2008-9-5 · A study of alginate lyase was carried out to determine if this enzyme could be used to remove alginate present in the core of alginate/poly-l-lysine (AG/PLL) microcapsules in order to maximize cell growth and colonization.A complete kinetic study was undertaken, which indicated an optimal activity of the enzyme at pH 7–8, 50 °C, in the presence of Ca 2+.
contact supplierWhatsappDetection and Pharmacokinetics of Alginate ...
2018-6-14 · Preparation of AOs. Sodium alginate (5g) was gradually dissolved in 500ml of distilled water with heating at 50 C, and an aliquot (1ml) of alginate lyase solution (500mg/ml) was added to the alginate solu-tion. The reaction mixture was incubated at 40 C for 3d. During the incubation period, the same volume of fresh enzyme solution was added 3 ...
contact supplierWhatsappChem. Biochem. Eng. Q. (1) 125–131 (2019), Alginate …
2019-4-11 · 126 W. Zhang et al., Alginate Lyase of a Novel Algae Fermentation Strain, Chem. Biochem. Eng. Q.33 (1) 125–131 (2019), search of alginate lyase will promote the research of algae biodegradation and fuels production from al-gae. In addition, alginate oligosaccharides are the products of alginate after degradation by alginate lyase.
contact supplierWhatsappThree Alginate Lyases from Marine Bacterium …
2010-12-19 · Three alginate lyases (A, B, and C) from an alginate-degrading marine bacterium strain HZJ216 isolated from brown seaweed in the Yellow Sea of China and identified preliminarily as Pseudomonas fluorescens are purified, and their biochemical properties are described. Molecular masses of the three enzymes are determined by SDS-PAGE to be 60.25, 36, and …
contact supplierWhatsappThe Function of CBM32 in Alginate Lyase VxAly7B on the ...
2022-3-21 · Most of CBM32 exists stably in alginate lyase (Lyu et al., 2018; Yan et al., 2019), only a few of CBM32 spontaneously cleaved from the alginate lyase, such as alginate lyase in Vibrio sp. QY102 (Sun et al., 2019) and VxAly7B in V. xiamenensis QY104.
contact supplierWhatsapp(PDF) Isolation of alginate lyase-producing actinomycetes ...
Isolation of alginate lyase-producing actinomycetes from the marine environment and purification of lyase ... The activities of 18 A.L.-producing strains were measured using liquid culture media ...
contact supplierWhatsappIsolation of a novel alginate lyaseâ producing Bacillus ...
effects. Alginate oligosaccharide is believed to be one of the important components to enhance growth and crop yield. In this study, we isolated and characterized a Bacillus litoralisBacillus strain, named M3, from decayed kelps. We further demonstrated that the M3 strain could secrete alginate lyase to degrade alginate.
contact supplierWhatsappCharacterization of a new alginate lyase from newly ...
2013-1-1 · By using the alginate as the sole carbon source, ten alginate metabolizing strains were obtained. Among these strains, the one designated as S20 showed the highest productivity of alginate lyase, with 0.37 Uml −1 extracellular alginate lyase and 1.34 Uml −1 intracellular alginate lyase activity after 48-h fermentation in liquid alginate medium. . Analysis of the 16S rDNA on …
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2018-3-9 · These characteristics suggested that AlgA is an endolytic polyM-specific alginate lyase (EC 4.2.2.3). The products of alginate and polyM degradation by AlgA were purified and identified through fast protein liquid …
contact supplierWhatsappThe role of alginate lyases in the enzymatic ...
2017-9-1 · In this work, we have compared the carbohydrate content and the enzymatic saccharification of the brown algae Macrocystis pyrifera from Chile and Saccharina latissima from Norway.M.pyrifera contained 40% mannitol, 31% uronic acids and 15% glucose, while S.latissima contained 37% glucose, 30% uronic acids and 25% mannitol. Thus, the ratio between mannitol …
contact supplierWhatsappProteomic enzyme analysis of the marine fungus ... - Nature
2019-8-26 · The fermentations with algae contained significantly higher levels of PL7 alginate lyases (5–16%), as was expected based on the observed alginate lyase activity (Fig. 5b), but surprisingly also ...
contact supplierWhatsapp高效产褐藻胶裂解酶菌株产酶条件优化及降解寡糖结构 ...
关键词: 海星 褐藻胶裂解酶 盐单胞菌 寡糖 Abstract: A alginate-producing lyase bacterial strain AlgX2 was selected in the viscera of starfish Asteroidea sp. using sodium alginate as sole carbon source in order to screen biodegradable alginate lyase microbial strain which was identified to belong to the genus of Cobetia, named as Cobetia AlgX2, upon on the identification of ...
contact supplierWhatsappExpression and characterization of a bifunctional alginate ...
2018-8-9 · In this study, an endolytic alginate lyase, named Al163, was identified, cloned, and characterized from the Antarctic bacterium Pseudoalteromonas sp. NJ-21. Comparative sequence analysis showed that the predicted amino acid sequence encoded by al163 belongs to the polysaccharide lyase 6 (PL-6) family and has a molecular mass of about 80 kDa. . …
contact supplierWhatsappCharacterization of Alginate Lyase Activity on Liquid ...
The alginate lyase showed the highest activity, when hexamers were provided as substrate. This indicated that the alginate lyase best accommodates a …
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